Bioc 471a/571a -applied Molecular Genetics Fall 2001 -dr. Roger Miesfeld
نویسنده
چکیده
Generating transgenic mice using fertilized egg cells Purified experimental DNA is directly injected into the male pronucleus of a fertilized mouse egg using a microscope-mounted manipulator and microneedle. The three essential instruments for embryo injection are an inverted microscope with stage magnification of 400X, a micromanipulator-mounted negative pressure holding pipette (left) and a micromanipulator-mounted injection pipette (right).
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